발치와의 치유과정에서 교원질 및 비교원단백질의 분포에 관한 면역조직화학적 연구
Immunohistochemical Distribution of Type I Collagen and Noncollagenous Proteins During Socket Healing of the Rat
림채영, 조진호, 김명식, 정희숙,
소속 상세정보
림채영 ( ) - 경희대학교
조진호 ( ) - 경희대학교
김명식 ( ) - 경희대학교
정희숙 ( ) - 경희대학교
KMID : 0355819950190010093
Abstract
During tooth socket healing, the coagulum in the socket is replaced by fibrous connective tissue which undergoes mineralization and eventually becomes bone. Using this model, the healing process of the tooth socket and the role of type I
collagen(CI),
fibronectin(FN), bone sialoprotein(BSP), and osteopontin(OP) in the process were studied. Thirty Sprague-Dawley rats weighing 130-150gm were fed β-aminopropionitrile for 5days before extraction of the first maxillary molars, and sacrified by
perfusion
with 4% paraformaldehyde at 1, 3, 5, 7, and 10 days after tooth extraction.
The socket and surrounding tissues were cut, demineralized, dehydrated and embedded in paraffin.
For morphological observation, the sections were stained by Azan, Immunostaining of the ECM components was achieved by the avidin-biotin complex method.
@ES The results as follows ;
@EN 1. Morpology of the tooth socket 1 day after tooth extraction. The socket was filled with blood coagulum which was composed of densly aggregated red blood, cells, platelets and fibrin network.
2. Morphology of the tooth socket 3days after tooth exytaction. A large portion of the coagulum was replaced by fibrous connective tissue contains a large number of fibroblasts, come from periphery of the socket.
3. At 5days, the newly formed bone demonstrated the highest proliferation activity. At 7days, the soclet was occupied with new bone.
4. Type I collagen was observed in the newly formed connective tissue and around the new bone, frber.
5. Intensive staining for fibronectin was observed in granulation tissue, especially around blood vessels as well as inflammatory cells.
6. Strong immunostaining for bone sialoprotein and osteopontin was found in osteoblasts and new bone, while weaker staining was observed on dense connective tissue.
7. There data suggest that collagen and noncollagen proteins (FN, BSP, OP) an improtant role daring socket healing.
키워드
원문 및 링크아웃 정보
등재저널 정보